Search or filter publications

Search publications Search

Filter by type:

Filter by publication type

• BOOK
• BOOK CHAPTER
• CONFERENCE PAPER
• JOURNAL ARTICLE
• PATENT
• REPORT
• SOFTWARE

Filter by year:

Filter from 201920182017201620152014201320122011201020092008 to Filter to 201920182017201620152014201320122011201020092008

---

Search results

• JOURNAL ARTICLE
  Kelwick RJR, Ricci L, Chee SM, Bell D, Webb A, Freemont Pet al., 2019,

  Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics

  , Synthetic Biology, Vol: 3, ISSN: 2397-7000

  The polyhydroxyalkanoates (PHAs) are microbially-produced biopolymers that could potentially be used as sustainable alternatives to oil-derived plastics. However, PHAs are currently more expensive to produce than oil-derived plastics. Therefore, more efficient production processes would be desirable. Cell-free metabolic engineering strategies have already been used to optimise several biosynthetic pathways and we envisioned that cell-free strategies could be used for optimising PHAs biosynthetic pathways. To this end, we developed several Escherichia coli cell-free systems for in vitro prototyping PHAs biosynthetic operons, and also for screening relevant metabolite recycling enzymes. Furthermore, we customised our cell-free reactions through the addition of whey permeate, an industrial waste that has been previously used to optimise in vivo PHAs production. We found that the inclusion of an optimal concentration of whey permeate enhanced relative cell-free GFPmut3b production by ∼50%. In cell-free transcription-translation prototyping reactions, GC-MS quantification of cell-free 3-hydroxybutyrate (3HB) production revealed differences between the activities of the Native ΔPhaC_C319A (1.18 ±0.39 µM), C104 ΔPhaC_C319A (4.62 ±1.31 µM) and C101 ΔPhaC_C319A (2.65 ±1.27 µM) phaCAB operons that were tested. Interestingly, the most active operon, C104 produced higher levels of PHAs (or PHAs monomers) than the Native phaCAB operon in both in vitro and in vivo assays. Coupled cell-free biotransformation/transcription-translation reactions produced greater yields of 3HB (32.87 ±6.58 µM) and these reactions were also used to characterise a Clostridium propionicum Acetyl-CoA recycling enzyme. Together, these data demonstrate that cell-free approaches complement in vivo workflows for identifying additional strategies for optimising PHAs production.

  • Abstract
  • Open Access Link
  • Cite
• JOURNAL ARTICLE
  Yu J, Knoppova J, Michoux F, Bialek W, Cota E, Shukla MK, Straskova A, Aznar GP, Sobotka R, Komenda J, Murray JW, Nixon PJet al., 2018,

  Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 115, Pages: E7824-E7833, ISSN: 0027-8424
  • Author Web Link
  • Open Access Link
  • Cite
• JOURNAL ARTICLE
  Johnson R, Ravenhall M, Pickard D, Dougan G, Byrne A, Frankel Get al., 2018,

  Comparison of Salmonella enterica Serovars Typhi and Typhimurium Reveals Typhoidal Serovar-Specific Responses to Bile

  , INFECTION AND IMMUNITY, Vol: 86, ISSN: 0019-9567
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 4
• JOURNAL ARTICLE
  Salih O, He S, Planamente S, Stach L, MacDonald JT, Manoli E, Scheres SHW, Filloux A, Freemont PSet al., 2018,

  Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa

  , STRUCTURE, Vol: 26, Pages: 329-+, ISSN: 0969-2126
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 3
• JOURNAL ARTICLE
  Bernal P, Llamas MA, Filloux A, 2018,

  Type VI secretion systems in plant-associated bacteria

  , ENVIRONMENTAL MICROBIOLOGY, Vol: 20, Pages: 1-15, ISSN: 1462-2912
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 9
• JOURNAL ARTICLE
  Aramayo RJ, Willhoft O, Ayala R, Bythell-Douglas R, Wigley DB, Zhang Xet al., 2018,

  Cryo-EM structures of the human INO80 chromatin-remodeling complex

  , NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 25, Pages: 37-+, ISSN: 1545-9993
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 8
• JOURNAL ARTICLE
  Mullineaux-Sanders C, Collins JW, Ruano-Gallego D, Levy M, Pevsner-Fischer M, Glegola-Madejska IT, Sagfors AM, Wong JLC, Elinav E, Crepin VF, Frankel Get al., 2017,

  Citrobacter rodentium Relies on Commensals for Colonization of the Colonic Mucosa

  , CELL REPORTS, Vol: 21, Pages: 3381-3389, ISSN: 2211-1247
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 4
• JOURNAL ARTICLE
  Portaliou AG, Tsolis KC, Loos MS, Balabanidou V, Rayo J, Tsirigotaki A, Crepin VF, Frankel G, Kalodimos CG, Karamanou S, Economou Aet al., 2017,

  Hierarchical protein targeting and secretion is controlled by an affinity switch in the type III secretion system of enteropathogenic Escherichia coli

  , EMBO JOURNAL, Vol: 36, Pages: 3517-3531, ISSN: 0261-4189
  • Author Web Link
  • Cite
  • Citations: 13
• JOURNAL ARTICLE
  Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li Het al., 2017,

  Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 114, Pages: E9529-E9538, ISSN: 0027-8424
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 8
• JOURNAL ARTICLE
  Berger CN, Crepin VF, Roumeliotis TI, Wright JC, Carson D, Pevsner-Fischer M, Furniss RCD, Dougan G, Dori-Bachash M, Yu L, Clements A, Collins JW, Elinav E, Larrouy-Maumus GJ, Choudhary JS, Frankel Get al., 2017,

  Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo

  , CELL METABOLISM, Vol: 26, Pages: 738-+, ISSN: 1550-4131
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 5
• JOURNAL ARTICLE
  Wen KY, Cameron L, Chappell J, Jensen K, Bell DJ, Kelwick R, Kopniczky M, Davies JC, Filloux A, Freemont PSet al., 2017,

  A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.

  , ACS Synthetic Biology, Vol: 6, Pages: 2293-2301, ISSN: 2161-5063

  Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC-MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.

  • Abstract
  • Open Access Link
  • Cite
• JOURNAL ARTICLE
  Cepeda-Molero M, Berger CN, Walsham ADS, Ellis SJ, Wemyss-Holden S, Schueller S, Frankel G, Angel Fernandez Let al., 2017,

  Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors

  , PLOS PATHOGENS, Vol: 13, ISSN: 1553-7366
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 3
• JOURNAL ARTICLE
  Beckova M, Yu J, Krynicka V, Kozlo A, Shao S, Konik P, Komenda J, Murray JW, Nixon PJet al., 2017,

  Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria

  , PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 372, ISSN: 0962-8436
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 5
• JOURNAL ARTICLE
  Karampatzakis A, Song CZ, Allsopp LP, Filloux A, Rice SA, Cohen Y, Wohland T, Torok Pet al., 2017,

  Probing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imaging

  , NPJ BIOFILMS AND MICROBIOMES, Vol: 3, ISSN: 2055-5008
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 5
• JOURNAL ARTICLE
  Sandu P, Crepin VF, Drechsler H, McAinsh AD, Frankel G, Berger CNet al., 2017,

  The Enterohemorrhagic Escherichia coli Effector EspW Triggers Actin Remodeling in a Rac1-Dependent Manner

  , INFECTION AND IMMUNITY, Vol: 85, ISSN: 0019-9567
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 2
• JOURNAL ARTICLE
  Heeb S, Camara M, Filloux A, Williams Pet al., 2017,

  Professor Dieter Haas (1945-2017) OBITUARY

  , FEMS MICROBIOLOGY REVIEWS, Vol: 41, Pages: 597-598, ISSN: 0168-6445
  • Author Web Link
  • Cite
• JOURNAL ARTICLE
  Stach L, Freemont PS, 2017,

  The AAA+ ATPase p97, a cellular multitool

  , BIOCHEMICAL JOURNAL, Vol: 474, Pages: 2953-2976, ISSN: 0264-6021
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 12
• JOURNAL ARTICLE
  Jakobsen TH, Warming AN, Vejborg RM, Moscoso JA, Stegger M, Lorenzen F, Rybtke M, Andersen JB, Petersen R, Andersen PS, Nielsen TE, Tolker-Nielsen T, Filloux A, Ingmer H, Givskov Met al., 2017,

  A broad range quorum sensing inhibitor working through sRNA inhibition

  , SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 9
• JOURNAL ARTICLE
  Witcomb LA, Czupryna J, Francis KP, Frankel G, Taylor PWet al., 2017,

  Non-invasive three-dimensional imaging of Escherichia coli K1 infection using diffuse light imaging tomography combined with micro-computed tomography

  , METHODS, Vol: 127, Pages: 62-68, ISSN: 1046-2023
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 2
• JOURNAL ARTICLE
  Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux Aet al., 2017,

  RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 114, Pages: 7707-7712, ISSN: 0027-8424
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 14
• JOURNAL ARTICLE
  Glyde R, Ye F, Darbari VC, Zhang N, Buck M, Zhang Xet al., 2017,

  Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation

  , MOLECULAR CELL, Vol: 67, Pages: 106-+, ISSN: 1097-2765
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 6
• JOURNAL ARTICLE
  Imbert PRC, Louche A, Luizet J-B, Grandjean T, Bigot S, Wood TE, Gagne S, Blanco A, Wunderley L, Terradot L, Woodman P, Garvis S, Filloux A, Guery B, Salcedo SPet al., 2017,

  A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors

  , EMBO JOURNAL, Vol: 36, Pages: 1869-1887, ISSN: 0261-4189
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 2
• JOURNAL ARTICLE
  Davies SK, Fearn S, Allsopp LP, Harrison F, Ware E, Diggle SP, Filloux A, McPhail DS, Bundy JGet al., 2017,

  Visualizing Antimicrobials in Bacterial Biofilms: Three-Dimensional Biochemical Imaging Using TOF-SIMS

  , MSPHERE, Vol: 2, ISSN: 2379-5042
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 2
• JOURNAL ARTICLE
  Smith WD, Bardin E, Cameron L, Edmondson CL, Farrant KV, Martin I, Murphy RA, Soren O, Turnbull AR, Wierre-Gore N, Alton EW, Bundy JG, Bush A, Connett GJ, Faust SN, Filloux A, Freemont PS, Jones AL, Takats Z, Webb JS, Williams HD, Davies JCet al., 2017,

  Current and future therapies for Pseudomonas aeruginosa infection in patients with cystic fibrosis

  , FEMS MICROBIOLOGY LETTERS, Vol: 364, ISSN: 0378-1097
  • Author Web Link
  • Cite
  • Citations: 6
• JOURNAL ARTICLE
  Willhoft O, McCormack EA, Aramayo RJ, Bythell-Douglas R, Ocloo L, Zhang X, Wigley DBet al., 2017,

  Crosstalk within a functional INO80 complex dimer regulates nucleosome sliding

  , ELIFE, Vol: 6, ISSN: 2050-084X
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 9
• JOURNAL ARTICLE
  McCarthy RR, Mazon-Moya MJ, Moscoso JA, Hao Y, Lam JS, Bordi C, Mostowy S, Filloux Aet al., 2017,

  Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion

  , NATURE MICROBIOLOGY, Vol: 2, ISSN: 2058-5276
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 8
• JOURNAL ARTICLE
  Pallett MA, Crepin VF, Serafini N, Habibzay M, Kotik O, Sanchez-Garrido J, Di Santo JP, Shenoy AR, Berger CN, Frankel Get al., 2017,

  Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells

  , MUCOSAL IMMUNOLOGY, Vol: 10, Pages: 602-612, ISSN: 1933-0219
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 13
• JOURNAL ARTICLE
  Bernal P, Allsopp LP, Filloux A, Llamas MAet al., 2017,

  The Pseudomonas putida T6SS is a plant warden against phytopathogens

  , ISME JOURNAL, Vol: 11, Pages: 972-987, ISSN: 1751-7362
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 23
• JOURNAL ARTICLE
  Pearson JS, Giogha C, Muhlen S, Nachbur U, Pham CLL, Zhang Y, Hildebrand JM, Oates CV, Lung TWF, Ingle D, Dagley LF, Bankovacki A, Petrie EJ, Schroeder GN, Crepin VF, Frankel G, Masters SL, Vince J, Murphy JM, Sunde M, Webb AI, Silke J, Hartland ELet al., 2017,

  EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation

  , NATURE MICROBIOLOGY, Vol: 2, ISSN: 2058-5276
  • Author Web Link
  • Cite
  • Citations: 20
• JOURNAL ARTICLE
  Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li Het al., 2017,

  Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1

  , NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 24, Pages: 316-+, ISSN: 1545-9993
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 25

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.